Toxicological Testing

Acute Toxicity

Undertaken by Insitute of Chemistry of Tallinn Technical University, Estonia.

Daphnia magna 24 hours acute toxity tests were carried out according to Finnish standard SFS 5062

The dilutions used were:

  • Anolyte medium : 1:100, 1:500 and 1:1000
  • Anolyte strong: 1:50, 1:200 and 1:500

The acute toxity of Anolyte

Anolyte type Dilution EC50; % (confidence limit) EC50; % for initial anolyte
Anolyte medium 1:100 25 (22.5-28) 0.25
  1:500 Not toxic  
  1:1000 Not toxic  
Anolyte strong 1:50 13 (11-16) 0.26-0.28
  1:200 56 (52-59)  
  1:500 Not toxic  

The toxity test demonstrated that there were no acute effects for the anolyte diluted 1:500 or more. No significant differences in the toxity of two anolytes (strong and medium) were observed.

Chronic Toxicity

Undertaken by Insitute of Chemistry of Tallinn Technical University, Estonia.

Protocol of the test

  • Daphnia magna was used for 21 day full life-cycle testing measuring two endpoints, mortality and reproduction.
  • Daphnia - clone used was of Finnish origin (EF) from the North Savo Regional Environmental Centre in Kuopio, Finland.
  • Five days old neonates were used to begin the 21-day test ........ (Full report is available upon request)

Results of the experiments

The concentrations used for the chronic test were ‹ MIC for D. magna. The maximum mortality observed was 6,7% (that means that one animal of 15 died during the 21 days test in the test vessel). No mortalities and no immobility were observed in any other tests. So it can be concluded that there were no significant differences in D. magna 21-day mortality between the tested water and control. Calculations made for the reproduction test included only survivors.

Conclusion No significant differences were found in the reproduction of Daphnia magna in all tested media. The mortality of Daphnias in the tests was less than 10% for sample (only one Daphnia died during the experiments). There were no differences observed in the size and weight at the endpoint of experiments between Daphnia magna grown in the tested samples and the control.

The results of the current study showed clearly that for both anolyte medium and anolyte strong no chronic effects were observed.